AsahiKASEI Glycated Albumin reagent

 

Overview

Lucica® GA-L is supplied as a ready-to-use, liquid reagent kit, for the measurement of glycated albumin, which contains high-specificity protease and enzymes, and can be utilized with general biochemical automated analyzers, and delivers same-visit results prior to physician consultation, and employs a novel BCP method that is highly specific to albumin.

 

Assay Principle

Glycated albumin: Influence by endogenous glycated amino acids is avoided through the use of an elimination reaction.

Glycated albumin: Influence by endogenous glycated amino acids is avoided through the use of an elimination reaction.

 

 

Albumin: A novel BCP method permits more specific measurement of albumin.

Albumin: A novel BCP method permits more specific measurement of albumin.

 

Calculation of glycated albumin (GA) value

Calculation of glycated albumin (GA) value

Kouzuma T, et al. Clinical Chimica Acta 346:135-143 (2004)

 

Assay Precision

Intra-day reproducibility(n=20)

  Control Normal sera Diabetic sera
Mean 14.4% 15.9% 24.5%
SD 0.09 0.13 0.23
CV 0.63% 0.82% 0.93%

 

Inter-day reproducibility(n=20)

  Normal sera Diabetic sera
Mean 16.2% 24.8%
SD 0.091 0.166
CV 0.56% 0.67%

 

Reduced Frequency of Calibration

 
  Normal sera Diabetic sera
Mean 15.9% 24.3%
SD 0.109 0.182
CV 0.68% 0.75%

A CV of less than 1% was attained even though calibration was conducted only on the first day of a two-week period of assays.

 

Interfering Substances

             Bilirubin F interference                     Bilirubin C interference

Bilirubin F interferenceBilirubin C interference

               Chyle interference                           Glucose interference

Chyle interferenceGlucose interference

            Ascorbic acid interference                Hemoglobin interference

Ascorbic acid interferenceHemoglobin interference

Bilirubin F and C, chyle, and glucose demonstrated almost no interference in the glycated albumin (GA) assay.

Ascorbic acid up to 100 mg/dL and hemoglobin up to 196 mg/dL demonstrated no interference in the GA assay.

Hemoglobin demonstrated slightly negative interference.

 

Anti-coagulant/Glycolytic Inhibitor Interference

Anti-coagulant/Glycolytic Inhibitor Interference

 

Linearity

Glycated albumin (g/dl)Albumin (g/dl)

 

Correlation

Correlation

 

Assay Examples

Glycated Albumin Reagents

Glycated Albumin Reagents

 

Albumin Reagents

Albumin Reagents